Exploring the effects of indole butyric acid (IBA) on in vitro growth of potato (Solanum tuberosum)

Abstract

In this study the effect of different concentration of indole butyric acid (IBA) was examined on in vitro propagation of potato (Solanum tuberosum). Three doses of IBA (1 ml, 2 ml, and 3 ml) were applied in liquid medium to potato explants of Asterix variety. The main purpose of this study was to optimize the dosage of IBA that would develop the best results for potato micropropagation. The experiments were conducted at Gilgit Baltistan's Agriculture Lab. Various parameters such as number of roots, roots length, and total weight were measured after exposing Asterix explants to varying concentrations of IBA. All tests were conducted under aseptic conditions. Analysis of variance (ANOVA) was used for analyzing the collected data. Throughout the study period, the results indicated significant differences (p>0.05) in potato growth among different concentrations of IBA. Among the different treatments, the Asterix potato variety exhibited the longest root length (11.1 cm) treated with 1 mL of IBA compared to the other concentrations. The IBA dose of 1 ml also proved to be the most effective in promoting the number of roots (9) in the Asterix variety. In this experimentation, 1 ml of indole-3-butyric acid (IBA) addition to the solution resulted in a major rise in the number of leaves (9) in Asterix plants. These findings have significant implications for future micropropagation and cultivation of potato plants. The ideal concentration of 1 ml of indole butyric acid (IBA) to promote root population can be used to develop effective and inexpensive protocols for in vitro potato propagation. © 2021 The Author(s)